Wang Keming Yongguo Shi  Jie Ding  YunTian Li, Wang YanLin  ZhaoxiaWang  Juan Li

Nanjing, China Second Affiliated Hospital, NanjingMedical UniversityDepartment of Oncology

Objective：BRAFactivated non-coding RNA (BANCR), a long non-coding RNA (lncRNA), is crucialfor cell migration in melanoma cells and non-small cell lung cancer (NSCLC)cells. However, little is known about the roles of this gene in theproliferation of colorectal cancer. Thus, we investigated the involvement ofBANCR in the proliferation of colorectal cancer. In this study, we show thatBANCR expression was significantly down-regulated in CRC tissues compared withnormal tissues, over expression of BANCR could suppresss CRC cell growth invitro and in vivo. We also found that pCDNA-BANCR-mediated CRC cell proliferationwas associated with induction of G0/G1 cell-cycle arrest and apoptosisenhancement through regulation of p21, and its effects were likelyposttranscriptional. Taken together, our findings suggest that down-regulatedof BANCR contributes to the proliferation of CRC cells, at least in part, throughthe regulation of p21 protein. Method：BRAFactivated non-coding RNA (BANCR), a long non-coding RNA (lncRNA), is crucialfor cell migration in melanoma cells and non-small cell lung cancer (NSCLC)cells. However, little is known about the roles of this gene in theproliferation of colorectal cancer. Thus, we investigated the involvement ofBANCR in the proliferation of colorectal cancer. In this study, we show thatBANCR expression was significantly down-regulated in CRC tissues compared withnormal tissues, over expression of BANCR could suppresss CRC cell growth invitro and in vivo. We also found that pCDNA-BANCR-mediated CRC cellproliferation was associated with induction of G0/G1 cell-cycle arrest andapoptosis enhancement through regulation of p21, and its effects were likelyposttranscriptional. Taken together, our findings suggest that down-regulatedof BANCR contributes to the proliferation of CRC cells, at least in part, throughthe regulation of p21 protein. Result：BRAFactivated non-coding RNA (BANCR), a long non-coding RNA (lncRNA), is crucialfor cell migration in melanoma cells and non-small cell lung cancer (NSCLC)cells. However, little is known about the roles of this gene in theproliferation of colorectal cancer. Thus, we investigated the involvement ofBANCR in the proliferation of colorectal cancer. In this study, we show thatBANCR expression was significantly down-regulated in CRC tissues compared withnormal tissues, overexpression of BANCR could suppresss CRC cell growth invitro and in vivo. We also found that pCDNA-BANCR-mediated CRC cellproliferation was associated with induction of G0/G1 cell-cycle arrest andapoptosis enhancement through regulation of p21, and its effects were likelyposttranscriptional.Taken together, our findings suggest that down-regulated ofBANCR contributes to the proliferation of CRC cells, at least in part, throughthe regulation of p21 protein. Conclusion：BRAFactivated non-coding RNA (BANCR), a long non-coding RNA (lncRNA), is crucialfor cell migration in melanoma cells and non-small cell lung cancer (NSCLC)cells. However, little is known about the roles of this gene in theproliferation of colorectal cancer. Thus, we investigated the involvement ofBANCR in the proliferation of colorectal cancer. In this study, we show thatBANCR expression was significantly down-regulated in CRC tissues compared withnormal tissues, overexpression of BANCR could suppresss CRC cell growth invitro and in vivo. We also found that pCDNA-BANCR-mediated CRC cellproliferation was associated with induction of G0/G1 cell-cycle arrest andapoptosis enhancement through regulation of p21, and its effects were likelyposttranscriptional. Taken together, our findings suggest that down-regulatedof BANCR contributes to the proliferation of CRC cells, at least in part，throughthe regulation of p21 protein.

Key Words：CRC